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Illustration of missing wedge in 2D, using a model sarcomere
a b c d e
|(a) Schematic muscle sarcomere with
Fourier transform in the inset above.
(b) High-pass filtered resulting from the Fourier transform filtered with the mask above.
(c) Low-pass filtered. This results in blurring fine detail.
(d) Wedge-filtered, illustrating the case in the electron microscope with the tilt axis along the sarcomere axis. Filaments are clearly resolved but perpendicular elements (M-line) are suppressed.
(e) Wedge-filtered, tilt axis now perpendicular to the sarcomere axis. Now all filaments are suppressed but M-line is clearly resolved.
For this example, dual-axis tilt-series as commonly used in electron tomography is homologous to combining both the wedge-filters, hence the original image would be obtained.